non-viral transfection methods optimized for gene delivery to a lung cancer cell line

Non-Viral Transfection Methods Optimized for Gene Delivery to a Lung Cancer Cell Line

BACKGROUND Mehr-80 is a newly established adherent human large cell lung cancer cell line that has not been transfected until now. This study aims to define the optimal transfection conditions and effects of some critical elements for enhancing gene delivery to this cell line by utilizing different non-viral transfection Procedures. METHODS In the current study, calcium phosphate (CaP), DEAE-...

Investigating lipopolymers based on polyethylenimine and nanoliposome for gene delivery to prostate cancer (PC3) cell line

Background: Non-viral Nano carriers such as liposomes and cationic polymers based on engineered properties are regarded in gene delivery field. Although these carriers do not have weaknesses of viral vectors, but they are less efficient than viruses and they still need to be improved as favorable gene delivery carriers. Amongst non-viral carriers, cationic liposomes have been proposed for clini...

in vivo transfection study of chitosan-dna-fap-b nanoparticles as a new non viral vector for gene delivery to the lung

Comparison of Commercial Transfection Reagents: Cell line optimized transfection kits for in vitro cancer research

The process of in vitro transfection involves introduction of genetic material into cells and it is generally used for mammalian cells and involves non-viral methods [1]. Various therapeutic cargo molecules can be used for intracellular delivery into cancer cell lines and primary cells, including plasmid DNA (pDNA), proteins, small molecules, messenger RNA (mRNA), shall RNA such as short interf...

Inorganic coatings for optimized non-viral transfection of stem cells

"Biomimetic" approaches for heterogeneous growth of inorganic coatings have become particularly widespread in biomedical applications, where calcium phosphate (CaP) mineral coatings are used to improve biomedical implants. Changes in coating properties can influence the effects of mineral coatings on adjacent cells, but to date it has not been practical to systematically vary inorganic coating ...

Targeted, non-viral gene delivery for cancer gene therapy.

The ability to mediate targeted and specific delivery of therapeutics to cancer cells remains one of the most important hurdles in effectively treating cancer. This aspect also remains as one of the greatest limitations of gene therapy as well. Targeted vectors based on the use of DNA-binding agents attached to cell specific ligands or "molecular conjugates" were created with the goal of over-c...